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Aggrecan-positive mesenchymal cells on tendon fibres are crucial for early tendon healing.
Tendon to bone healing is considered to be a chronic process due to low mechanical properties of adult bone and tendon. Recent studies demonstrated a small population of tendon cells with a phenotype of non-chondrogenic type II cell of which are potential progenitors and contribute to successful tendon to bone healing in some histological stages of tendon to bone healing, or tendon healing. However, the mechanisms by which tendon to bone healing occurs remain largely unknown. In this study, we focused on hyaline-like fracture healing at the early phase of tendon to bone healing of mice, with the question of whether the tendon cell is essential for fracture healing. We utilized the transgenic mouse line of Cre recombinase fused with a fluorescence protein, a recombination-dependent Cre, or rtTA. The source of mesenchymal cells was restrictedly examined by using rtTA; expressing Cre recombinase fused with a fluorescence protein (Mesenchyme) and rtTA; expressing Cre recombinase fused with a fluorescence protein (Fibroblast). In the in vivo model of the fracture healing of rtTA/R26R; Rosa mT/mG mice, we demonstrated that aggregates of glial fibrillary acidic protein (GFAP)-positive cells on tendon fibres could be observed at 12 hours after fracture in wild-type mice (WT) only. By contrast, we found a population of GFP-positive cells on tendon fibres at 12 hours after fracture in rtTA/R26R; Rosa m
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