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The analysis of all-rac-alpha-tocopherol in vegetable oils by HPLC/ESI-MS was investigated. The determination of alpha-tocopherol was carried out by reversed phase chromatographic separation on a chiral column using a mobile phase with a combination of methanol and hexane (30:70 v/v). The molecular ions of alpha-tocopherol and internal standard, all-rac-beta-tocopherol were subjected to ESI-MS. Under atmospheric pressure chemical ionization conditions, alpha-tocopherol showed a dominant [M – H](- ) deprotonated ion in its mass spectrum. The relative standard deviation of retention time and peak area of alpha-tocopherol in oils was less than 4.0% and 1.8%, respectively. The calibration curve of alpha-tocopherol showed good linearity over the concentration range of 5.0-20.0 microg/ml (R(2) = 0.999). The limit of detection was 3.1 ng/ml. The method was applied to the analysis of all-rac-alpha-tocopherol in several vegetable oils.Heparin-induced thrombocytopenia.
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