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Oxidative stress is a common pathogenic factor in acute ischemic stroke. N-acetyl L-cysteine (NAC) is a well-known mucolytic agent. It acts as a potent free radical scavenger in a variety of inflammatory diseases. The neuroprotective effects of NAC in acute ischemic stroke are still unclear. The purpose of this study was to investigate the neuroprotective effects of NAC at a low dose on mitochondria dysfunction and oxidative stress in experimental focal cerebral ischemia-reperfusion (I/R) in rats. We investigated the effects of NAC on mitochondrial parameters, oxidative stress and infarct volume in rats submitted to middle cerebral artery occlusion (MCAO) for 2 h followed by reperfusion. NAC was administered intraperitoneally at a dose of 100 mg/kg and MCAO/reperfusion. N-acetyl-L-cysteine has a neuroprotective effect, reducing the infarct volume and improving mitochondrial parameters in the striatum. The effects of NAC, however, were more pronounced when it was administered a day before the MCAO. The antioxidant effects of NAC are dose dependent, and they do not appear to be related to the inhibition of inflammatory mediators, since the levels of oxidative stress parameters and inflammatory markers were not significantly altered. Our data suggest that NAC could be useful in the treatment of acute ischemic stroke, and that its neuroprotective mechanism probably involves the activation of antioxidant defense systems, particularly in the non-ischemic hemisphere. FILED
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